6M58

Crystal structure of a complex between human serum albumin and the antibody Fab SL335

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-23
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	68.607, 103.931, 161.082
Unit cell angles	90.00, 93.08, 90.00

Refinement procedure

Resolution	30.000 - 2.950
Rwork	0.193
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5csz 1ao6
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.950	2.950
Rmerge	0.101	0.565
Number of reflections	47902	2445
<I/σ(I)>	15.4	2.3
Completeness [%]	98.6	99.5
Redundancy	3.1	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 4000, Tris, pH 8.5, glycerol, sodium acetate