6M4T

U shaped head to head four-way junction in d(TTCTGCTGCTGAA) sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-01
Detector	RAYONIX MX300HE
Wavelength(s)	0.91932
Spacegroup name	P 1 21 1
Unit cell lengths	57.204, 62.631, 29.743
Unit cell angles	90.00, 121.29, 90.00

Refinement procedure

Resolution	19.734 - 1.550
R-factor	0.1675
Rwork	0.165
R-free	0.19000
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.251
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.610
High resolution limit [Å]	1.543	3.340	1.550
Rmerge	0.042	0.025	0.604
Rmeas	0.050	0.029	0.714
Rpim	0.026	0.015	0.377
Number of reflections	13080	1321	1250
<I/σ(I)>	16.1
Completeness [%]	99.2	98.4	95.3
Redundancy	3.7	3.6	3.4
CC(1/2)		0.999	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	MES (pH=6.5) 50mM, MPD 35 %, Potassium chloride 200mM, CoCl2 20mM