6M4F
Crystal structure of the E496A mutant of HsBglA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.751, 67.617, 200.867 |
| Unit cell angles | 90.00, 104.07, 90.00 |
Refinement procedure
| Resolution | 48.710 - 2.200 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.18620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gxp |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.710 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.059 | 0.641 |
| Number of reflections | 127314 | 12641 |
| <I/σ(I)> | 14.54 | 2 |
| Completeness [%] | 99.4 | 99.58 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.999 | 0.685 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M SPG Buffer pH 7.0, 27% (w/v) PEG 1500 |
