6M3V
355 bp di-nucleosome harboring cohesive DNA termini
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2019-08-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.987, 228.970, 118.839 |
| Unit cell angles | 90.00, 92.68, 90.00 |
Refinement procedure
| Resolution | 49.330 - 4.600 |
| R-factor | 0.2061 |
| Rwork | 0.205 |
| R-free | 0.26050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ut9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.282 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 4.920 |
| High resolution limit [Å] | 4.600 | 4.600 |
| Rmerge | 0.129 | 1.800 |
| Rpim | 0.054 | 0.818 |
| Number of reflections | 26415 | 4782 |
| <I/σ(I)> | 6.6 | 1.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 6.6 |
| CC(1/2) | 0.995 | 0.481 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 291 | Calcium chloride, Potassium chloride, Potassium Cacodylate, Poly glutamic acid |
