6M3T
Crystal structure of the mouse endonuclease EndoG(H138A/C110A), space group P41212
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 198 |
Detector technology | CCD |
Collection date | 2016-03-09 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 68.594, 68.594, 251.321 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.250 - 2.380 |
R-factor | 0.2044 |
Rwork | 0.202 |
R-free | 0.24370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6m3f |
RMSD bond length | 0.003 |
RMSD bond angle | 0.593 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.250 | 2.470 |
High resolution limit [Å] | 2.380 | 2.380 |
Number of reflections | 25056 | 25056 |
<I/σ(I)> | 15.68 | |
Completeness [%] | 99.6 | |
Redundancy | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 13% PEG 8000, 0.1M HEPES at pH 7.5, 15% ethylene glycol, 7% Dimethyl sulfoxide |