6M3T

Crystal structure of the mouse endonuclease EndoG(H138A/C110A), space group P41212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	198
Detector technology	CCD
Collection date	2016-03-09
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	68.594, 68.594, 251.321
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.250 - 2.380
R-factor	0.2044
Rwork	0.202
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m3f
RMSD bond length	0.003
RMSD bond angle	0.593
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.250	2.470
High resolution limit [Å]	2.380	2.380
Number of reflections	25056	25056
<I/σ(I)>	15.68
Completeness [%]	99.6
Redundancy	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	13% PEG 8000, 0.1M HEPES at pH 7.5, 15% ethylene glycol, 7% Dimethyl sulfoxide