6M3Q
Crystal structure of AnkB/beta4-spectrin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97799 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.900, 110.485, 131.675 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.357 - 3.436 |
| R-factor | 0.2548 |
| Rwork | 0.252 |
| R-free | 0.30710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d8o 3kbt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.064 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.460 |
| High resolution limit [Å] | 3.400 | 9.210 | 3.400 |
| Rmerge | 0.255 | 0.047 | 0.767 |
| Rmeas | 0.277 | 0.051 | 0.835 |
| Rpim | 0.106 | 0.020 | 0.325 |
| Total number of observations | 94782 | ||
| Number of reflections | 14141 | 788 | 686 |
| <I/σ(I)> | 4.4 | ||
| Completeness [%] | 99.5 | 98.1 | 98.4 |
| Redundancy | 6.7 | 6.3 | 6.2 |
| CC(1/2) | 0.998 | 0.661 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 289 | 25% w/v pentaerythritol propoxylate 629 (17/8 PO/OH), 50 mM magnesium chloride and 0.1 M HEPES, pH 7.5 |
