6M3P
Crystal structure of AnkG/beta2-spectrin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 1 |
Unit cell lengths | 66.277, 66.305, 160.926 |
Unit cell angles | 99.47, 99.78, 100.87 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.360 |
High resolution limit [Å] | 3.300 | 8.940 | 3.300 |
Rmerge | 0.112 | 0.043 | |
Rmeas | 0.131 | 0.056 | |
Rpim | 0.088 | 0.035 | 0.891 |
Total number of observations | 103394 | ||
Number of reflections | 37513 | 1911 | 1815 |
<I/σ(I)> | 2.6 | ||
Completeness [%] | 97.7 | 99.6 | 97.7 |
Redundancy | 2.8 | 3 | 2.5 |
CC(1/2) | 0.802 | 0.566 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 289 | 10% w/v polyethylene glycol 6,000, 5% v/v (+/-)-2-methyl-2,4-pentanediol and 0.1 M HEPES pH 7.5 |