6M3H

Crystal structure of mouse HPF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-07
Detector	Nonius Kappa CCD
Wavelength(s)	0.97849
Spacegroup name	P 21 21 21
Unit cell lengths	42.031, 79.895, 106.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.360 - 1.710
R-factor	0.1838
Rwork	0.182
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m3g
RMSD bond length	0.006
RMSD bond angle	0.943
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.770
High resolution limit [Å]	1.710	1.710
Rpim	0.023	0.351
Number of reflections	39364	2485
<I/σ(I)>	31.1
Completeness [%]	99.3
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		300	0.1M Tris-pH8.3, 0.2 M Potassium citrate tribasic monohydrate, 20% w/v PEG 3350,