6M3G

Crystal structure of human HPF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-17
Detector	Nonius Kappa CCD
Wavelength(s)	0.97853
Spacegroup name	H 3 2
Unit cell lengths	89.981, 89.981, 226.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.860 - 1.570
R-factor	0.1891
Rwork	0.188
R-free	0.20480
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.942
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHARP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.570	1.570
Rpim	0.017
Number of reflections	49462	2567
<I/σ(I)>	45.2
Completeness [%]	99.3
Redundancy	9.6
CC(1/2)		0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		300	0.1 M Tris-pH 8.1, 0.2 M potassium acetate, 20% w/v Polyethylene glycol (PEG) 3350