6M33
Crystal structure of Homo Sapian GCP6 N-terminus and Mozart1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-28 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.99984 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.466, 69.466, 118.186 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.771 - 3.294 |
| R-factor | 0.229930059088 |
| Rwork | 0.222 |
| R-free | 0.29826 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.338 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.410 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Number of reflections | 5211 | 505 |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 98.2 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.997 | 0.695 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3.5M Ammonium chloride, 0.1M Bis-Tris propane p.H 7.0 |
