6M2L
Crystal structure of Plasmodium falciparum hexose transporter PfHT1 bound with C3361
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-12-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 102.432, 102.432, 248.305 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.959 - 3.700 |
| R-factor | 0.3412 |
| Rwork | 0.337 |
| R-free | 0.38230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zw9 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.714 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.900 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Number of reflections | 14879 | 2116 |
| <I/σ(I)> | 10.69 | |
| Completeness [%] | 99.8 | |
| Redundancy | 82.38 | |
| CC(1/2) | 0.998 | 0.869 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.2 | 293 | 0.1 M NH4Cl, 0.1 M Na3Citrate pH 5.2, 28% PEG500MME |
