6M2K

Uncommon structural features of rabbit MHC class I (RLA-A1) complexed with rabbit haemorrhagic disease virus (RHDV) derived peptide, VP60-10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-10-15
Detector	SDMS
Wavelength(s)	1.03923
Spacegroup name	P 21 21 21
Unit cell lengths	49.612, 74.303, 152.192
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.970 - 2.590
R-factor	0.219
Rwork	0.215
R-free	0.29380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5deg
RMSD bond length	0.008
RMSD bond angle	1.018
Data reduction software	MOSFLM
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.590	2.590
Rmerge	0.112	0.758
Number of reflections	18060	18060
<I/σ(I)>	2.78
Completeness [%]	99.9
Redundancy	11.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M BIS-TRIS PH 6.5, 20% w/v PEG 5000