6LZB
crystal structure of Human Methionine aminopeptidase (HsMetAP1b) in complex with AN-P2-5H-06
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.95360 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.573, 77.251, 48.623 |
| Unit cell angles | 90.00, 91.54, 90.00 |
Refinement procedure
| Resolution | 40.530 - 1.290 |
| R-factor | 0.1645 |
| Rwork | 0.163 |
| R-free | 0.19980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3h |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.145 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP (11.7.01) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.560 | 47.560 | 1.310 |
| High resolution limit [Å] | 1.290 | 7.070 | 1.290 |
| Rmerge | 0.047 | 0.038 | 0.972 |
| Rmeas | 0.051 | 0.042 | 1.065 |
| Rpim | 0.019 | 0.016 | 0.426 |
| Total number of observations | 580633 | 3584 | 25874 |
| Number of reflections | 87661 | 563 | 4267 |
| <I/σ(I)> | 17.9 | 46.2 | 2.2 |
| Completeness [%] | 99.7 | 99.4 | 98.5 |
| Redundancy | 6.6 | 6.4 | 6.1 |
| CC(1/2) | 0.999 | 0.998 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 0.1M Bistris pH-6.2, 19% PEG 3350, 5% Glycerol |
