6LYS

Structure of the BAM complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97919
Spacegroup name	P 41 21 2
Unit cell lengths	117.073, 117.073, 429.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.050
R-factor	0.2448
Rwork	0.243
R-free	0.28440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d0o
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.160
High resolution limit [Å]	3.050	3.050
Number of reflections	53217	3492
<I/σ(I)>	20.7
Completeness [%]	99.9
Redundancy	12.8
CC(1/2)	0.998	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	27-30% PEG 400, 100mM NaCl