6LYO

Crystal Structure of H296A mutant of Formylglycinamidine Synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2019-06-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5417
Spacegroup name	P 65
Unit cell lengths	146.288, 146.288, 140.957
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.880 - 1.870
R-factor	0.127
Rwork	0.125
R-free	0.15780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t3t
RMSD bond length	0.022
RMSD bond angle	2.171
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.870	1.870
Rpim	0.033
Number of reflections	140431	6924
<I/σ(I)>	27.4
Completeness [%]	99.7
Redundancy	8.1
CC(1/2)		0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2M Ammonium Sulphate