6LYO
Crystal Structure of H296A mutant of Formylglycinamidine Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-06-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5417 |
| Spacegroup name | P 65 |
| Unit cell lengths | 146.288, 146.288, 140.957 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.880 - 1.870 |
| R-factor | 0.127 |
| Rwork | 0.125 |
| R-free | 0.15780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3t |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rpim | 0.033 | |
| Number of reflections | 140431 | 6924 |
| <I/σ(I)> | 27.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 8.1 | |
| CC(1/2) | 0.926 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2M Ammonium Sulphate |
