6LYM

Crystal structure of D657A mutant of formylglycinamidine synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2016-05-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5417
Spacegroup name	P 65
Unit cell lengths	147.296, 147.296, 141.455
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.210 - 2.460
R-factor	0.1606
Rwork	0.158
R-free	0.21290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t3t
RMSD bond length	0.015
RMSD bond angle	1.817
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.460	2.460
Rmeas	0.155
Number of reflections	59677	3022
<I/σ(I)>	13.6
Completeness [%]	94.6
Redundancy	6
CC(1/2)		0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2M Ammonium Sulphate