6LXU
Crystal structure of methionine gamma-lyase from Fusobacterium nucleatum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2015-12-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 65.947, 99.915, 138.297 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.780 - 1.190 |
R-factor | 0.15243 |
Rwork | 0.152 |
R-free | 0.16101 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rfv |
RMSD bond length | 0.008 |
RMSD bond angle | 1.419 |
Data reduction software | DIALS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.060 | 1.220 |
High resolution limit [Å] | 1.190 | 1.190 |
Rmerge | 0.060 | 0.250 |
Rmeas | 0.064 | 0.285 |
Rpim | 0.017 | 0.097 |
Number of reflections | 145156 | 10334 |
<I/σ(I)> | 26.3 | 6.7 |
Completeness [%] | 99.7 | 96.8 |
Redundancy | 13.5 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 41% (v/v) 2-methyl-2,4-pentanediol (MPD), 0.2M Ammonium nitrate |