6LXT

Structure of post fusion core of 2019-nCoV S2 subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-02-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	51.240, 57.580, 115.720
Unit cell angles	90.00, 91.58, 90.00

Refinement procedure

Resolution	47.320 - 2.900
Rwork	0.259
R-free	0.28960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wyy
RMSD bond length	0.013
RMSD bond angle	1.933
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.320	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.160	1.130
Number of reflections	14470	1518
<I/σ(I)>	6.3
Completeness [%]	94.0
Redundancy	2
CC(1/2)	1.000	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289	10% PEG 8000, 200 mM zinc acetate, 0.1 M MES, pH 6.0