6LXT
Structure of post fusion core of 2019-nCoV S2 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-02-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.240, 57.580, 115.720 |
| Unit cell angles | 90.00, 91.58, 90.00 |
Refinement procedure
| Resolution | 47.320 - 2.900 |
| Rwork | 0.259 |
| R-free | 0.28960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wyy |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.933 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.320 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.160 | 1.130 |
| Number of reflections | 14470 | 1518 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 94.0 | |
| Redundancy | 2 | |
| CC(1/2) | 1.000 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 10% PEG 8000, 200 mM zinc acetate, 0.1 M MES, pH 6.0 |
