6LXP
TvCyP2 in apo form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-05-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.823, 54.262, 59.855 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.130 - 2.350 |
R-factor | 0.1629 |
Rwork | 0.155 |
R-free | 0.22520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yba |
RMSD bond length | 0.007 |
RMSD bond angle | 0.899 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.130 | 2.441 |
High resolution limit [Å] | 2.350 | 2.356 |
Number of reflections | 6685 | 487 |
<I/σ(I)> | 16.8 | 2.8 |
Completeness [%] | 91.4 | 91.8 |
Redundancy | 4.5 | |
CC(1/2) | 0.970 | 0.919 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 288 | 0.1 M TRIS pH 8.5, 0.3 M Magnesium formate dihydrate |