6LXO
TvCyP2 in apo form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.975 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.811, 56.189, 59.804 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.090 - 1.890 |
R-factor | 0.1433 |
Rwork | 0.139 |
R-free | 0.17840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yba |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.090 | 1.959 |
High resolution limit [Å] | 1.890 | 1.891 |
Number of reflections | 14047 | 1363 |
<I/σ(I)> | 33.813 | 10.6 |
Completeness [%] | 98.2 | 90.9 |
Redundancy | 4.7 | |
CC(1/2) | 0.993 | 0.979 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 288 | 0.1 M Citric Acid pH 5.0, 1.6 M Ammonium Sulfate |