6LX1
Potato D-enzyme complexed with Acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-25 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.171, 120.293, 174.698 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.960 - 2.030 |
| R-factor | 0.16542 |
| Rwork | 0.163 |
| R-free | 0.20563 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x1n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.693 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.085 | 0.322 |
| Number of reflections | 46983 | 4620 |
| <I/σ(I)> | 32.3 | 7.9 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 | 293 | 12% PEG 8000, 100 mM HEPES, pH 7.6, 100 mM CaCl2 |
