6LUP
Crystal structure of shark MHC CLASS I for 2.3 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 64 |
| Unit cell lengths | 125.859, 125.859, 132.473 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.622 - 2.302 |
| Rwork | 0.255 |
| R-free | 0.28350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y91 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.362 |
| High resolution limit [Å] | 2.300 | 2.302 |
| Rmerge | 0.110 | 0.571 |
| Number of reflections | 52646 | 3679 |
| <I/σ(I)> | 21.185 | 4.267 |
| Completeness [%] | 99.8 | 99.38 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | pH 7.5, 0.09 M HEPES sodium, 1.26 M Sodium citrate tribasic dihydrate,10% v/v Glycerol |
