6LUN

NN2101 Antibody Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97960
Spacegroup name	P 1 21 1
Unit cell lengths	72.753, 69.702, 90.058
Unit cell angles	90.00, 107.71, 90.00

Refinement procedure

Resolution	36.531 - 1.900
R-factor	0.1667
Rwork	0.165
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bvp
RMSD bond length	0.007
RMSD bond angle	0.868
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.531	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.131
Rpim	0.047
Number of reflections	66823	3291
<I/σ(I)>	26
Completeness [%]	97.0
Redundancy	7.6
CC(1/2)		0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% (w/v) PEG 6000, 100mM HEPES-NaOH pH7.0, 200mM NaCl