6LUK

Crystal structure of the SAMD1 SAM domain in another crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2020-01-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97891
Spacegroup name	P 1 21 1
Unit cell lengths	66.430, 182.842, 66.971
Unit cell angles	90.00, 93.32, 90.00

Refinement procedure

Resolution	45.710 - 2.054
R-factor	0.1872
Rwork	0.185
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	A model solved by Se-Met labelled sample.
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.054	2.054
Rmerge	0.099	0.520
Number of reflections	98440	8178
<I/σ(I)>	12.1	1.9
Completeness [%]	99.9
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1M bis-tris (pH 7.5), 2.1M ammonium sulphate