6LTY
DNA bound antitoxin HigA3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.615, 101.812, 58.027 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 41.790 - 3.280 |
| R-factor | 0.2799 |
| Rwork | 0.277 |
| R-free | 0.32560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ltz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.277 | 3.277 |
| Rmeas | 0.072 | 0.654 |
| Number of reflections | 6656 | 316 |
| <I/σ(I)> | 27.3 | |
| Completeness [%] | 99.3 | |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 23% w/v PEG3350, 0.2 M sodium acetate trihydrate pH 7.0 |
