6LTG

Crystal structure of the Fab fragment of murine monoclonal antibody OHV-3 against Human herpesvirus 6B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-05
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	81.380, 95.200, 113.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.645 - 1.630
R-factor	0.1824
Rwork	0.182
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wcy
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.645	38.645	1.670
High resolution limit [Å]	1.630	7.290	1.630
Rmerge	0.059	0.022	0.710
Rmeas	0.070	0.027	0.835
Total number of observations	756090
Number of reflections	212352	2329	15724
<I/σ(I)>	13.4	39.45	1.85
Completeness [%]	99.6	98.1	99.8
Redundancy	3.561	3.275	3.646
CC(1/2)	0.999	0.999	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	100 mM BIS-Tris pH 6.5, 0.2 M MgCl2, polyethylene glycol 3350 23% (w/v)