6LSG

Crystal structure of the enterovirus 71 polymerase elongation complex (C0S6M form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	63.564, 77.220, 154.164
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.370 - 2.140
R-factor	0.1917
Rwork	0.190
R-free	0.21660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f8g
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.14)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.200
High resolution limit [Å]	2.120	4.570	2.120
Rmerge	0.031	0.024	0.201
Rmeas	0.034	0.026	0.227
Rpim	0.014	0.010	0.104
Total number of observations	262212
Number of reflections	42624	4647	3000
<I/σ(I)>	18.9
Completeness [%]	96.9	99.6	69.4
Redundancy	6.2	6	4.5
CC(1/2)		0.999	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	289	MES, PEG 5000 monomethyl ether, glycerol