6LSD

Crystal Structure of YEATS domain of human YEATS2 in complex with H3K27bz peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 4 2 2
Unit cell lengths	89.674, 89.674, 86.943
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.840 - 2.050
R-factor	0.1942
Rwork	0.191
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iql
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.090
High resolution limit [Å]	2.050	5.560	2.050
Rmerge	0.078	0.034	0.787
Rmeas	0.082	0.036	0.835
Rpim	0.026	0.012	0.274
Total number of observations	220973
Number of reflections	22927	1296	1132
<I/σ(I)>	6.3
Completeness [%]	100.0	100	100
Redundancy	9.6	8.5	9.1
CC(1/2)		0.999	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	291	0.1 M Bis-Tris, pH 9.0, 2.0M ammonium sulfate