6LSA
Complex structure of bovine herpesvirus 1 glycoprotein D and bovine nectin-1 IgV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-04 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97892 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.871, 64.234, 102.931 |
| Unit cell angles | 90.00, 91.78, 90.00 |
Refinement procedure
| Resolution | 45.196 - 2.204 |
| R-factor | 0.2013 |
| Rwork | 0.200 |
| R-free | 0.23620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5x5w |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.337 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.069 | 0.783 |
| Number of reflections | 58498 | 5751 |
| <I/σ(I)> | 23.9 | |
| Completeness [%] | 98.1 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 281 | 0.1M Sodium HEPES, 15% w/v Polyethylene glycol 4000 |
