6LS9
Crystal structure of bovine herpesvirus 1 glycoprotein D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.03645 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.272, 104.392, 98.772 |
| Unit cell angles | 90.00, 112.50, 90.00 |
Refinement procedure
| Resolution | 37.433 - 2.503 |
| R-factor | 0.2072 |
| Rwork | 0.205 |
| R-free | 0.24330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5x5v |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.205 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.573 |
| Number of reflections | 37572 | 3702 |
| <I/σ(I)> | 21.111 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 0.2M Ammonium acetate, 0.1M Tris-HCl, 16% w/v Polyethylene glycol 10000 |
