6LS6

Crystal Structure of YEATS domain of AF9 in complex with H3K9bz peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	90.258, 42.236, 90.253
Unit cell angles	90.00, 96.64, 90.00

Refinement procedure

Resolution	44.824 - 2.200
R-factor	0.2301
Rwork	0.227
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tmp
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14rc3_3206)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.196	5.970	2.200
Rmerge	0.139	0.077	0.422
Rmeas	0.159	0.088	0.492
Rpim	0.075	0.040	0.249
Number of reflections	16939	868	808
<I/σ(I)>	5.7
Completeness [%]	96.6	93.5	94
Redundancy	4	4.5	3.3
CC(1/2)		0.992	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.1 M Bis-Tris, pH 5.5??200 mM lithium sulfate, 25% w/v PEG 3350.