6LRU

Marsupenaeus japonicus ferritin mutant (T158H)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	273.15
Detector technology	CCD
Collection date	2019-05-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.4813
Spacegroup name	P 4 3 2
Unit cell lengths	117.349, 117.349, 117.349
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.109 - 2.400
R-factor	0.1358
Rwork	0.131
R-free	0.18340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a4u
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.398	5.170	2.400
Rmerge	0.237	0.099	0.722
Rmeas	0.241	0.100	0.732
Rpim	0.042	0.018	0.119
Number of reflections	20515	1277	1109
<I/σ(I)>	8.2
Completeness [%]	100.0	99.7	100
Redundancy	34.3	30.8	37.5
CC(1/2)		0.999	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	2000 mM NaCl, 100 mM imidazole (pH 8.0), 2 mM NiSO4