6LQE
Crystal structure of Arabidopsis ARID5 PHD finger in complex with H3K4me3 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 27.922, 27.922, 124.513 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.128 - 1.900 |
R-factor | 0.2026 |
Rwork | 0.201 |
R-free | 0.24090 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.107 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
Rmerge | 0.106 | 0.078 | 1.005 |
Rmeas | 0.108 | 0.080 | 1.030 |
Rpim | 0.023 | 0.019 | 0.220 |
Number of reflections | 4421 | 289 | 206 |
<I/σ(I)> | 5.7 | ||
Completeness [%] | 99.8 | 99 | 100 |
Redundancy | 22.3 | 17.8 | 21.5 |
CC(1/2) | 0.998 | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1 M sodium acetate, pH 4.6, 30% PEG 300 |