6LQ9

S109 in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	105.105, 105.105, 303.825
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.710 - 2.500
R-factor	0.1921
Rwork	0.190
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hat
RMSD bond length	0.008
RMSD bond angle	1.193
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.222	0.120
Rmeas	0.231	0.126
Rpim	0.085	0.037	0.680
Total number of observations	784766
Number of reflections	60570	3359	2950
<I/σ(I)>	1.9
Completeness [%]	100.0	99.9	99.9
Redundancy	13	11.8	11
CC(1/2)		0.994	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	18% PEG3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH6.6