6LQ9
S109 in complex with CRM1-Ran-RanBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 105.105, 105.105, 303.825 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.710 - 2.500 |
| R-factor | 0.1921 |
| Rwork | 0.190 |
| R-free | 0.22790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hat |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.193 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.222 | 0.120 | |
| Rmeas | 0.231 | 0.126 | |
| Rpim | 0.085 | 0.037 | 0.680 |
| Total number of observations | 784766 | ||
| Number of reflections | 60570 | 3359 | 2950 |
| <I/σ(I)> | 1.9 | ||
| Completeness [%] | 100.0 | 99.9 | 99.9 |
| Redundancy | 13 | 11.8 | 11 |
| CC(1/2) | 0.994 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 18% PEG3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH6.6 |
