6LPM

Crystal structure of AP endonuclease from Deinococcus radioduran

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	95
Detector technology	PIXEL
Collection date	2018-04-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	57.420, 58.860, 74.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.430 - 1.500
R-factor	0.1871
Rwork	0.186
R-free	0.20440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j5c
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.430	29.430	1.530
High resolution limit [Å]	1.495	6.690	1.500
Rmerge	0.066	0.055	0.473
Rmeas	0.074	0.062	0.561
Number of reflections	40705	523	2760
<I/σ(I)>	14.65	23.07	4.34
Completeness [%]	98.2	96.9	91
Redundancy	5.094	4.671	3.446
CC(1/2)	0.997	0.991	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Bis-Tris, PEG 5000