6LPK
A2AR crystallized in EROCOC17+4, LCP-SFX at 293 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2017-07-09 |
| Detector | MPCCD |
| Wavelength(s) | 1.230 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.500, 179.200, 142.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.400 - 1.800 |
| R-factor | 0.1786 |
| Rwork | 0.177 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eyi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.582 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 48924 | 2438 |
| <I/σ(I)> | 7.6 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 306.5 | 90.9 |
| CC(1/2) | 0.995 | 0.393 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100 mM sodium citrate pH5.5, 37% PEG300, 50 mM NaSCN, and 2% 2,5-hexanediol |
