6LPI

Crystal Structure of AHAS holo-enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	111.173, 104.577, 118.535
Unit cell angles	90.00, 105.68, 90.00

Refinement procedure

Resolution	35.929 - 2.849
R-factor	0.1697
Rwork	0.167
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jsc 2lvw
RMSD bond length	0.009
RMSD bond angle	1.302
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_2666)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.960
High resolution limit [Å]	2.849	2.850
Number of reflections	60768	5694
<I/σ(I)>	17.9
Completeness [%]	96.1
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	Tris-HCl, MgCl2, PEG 3350