6LPH
the Sufu-Fu complex crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.91878 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.145, 68.423, 56.144 |
| Unit cell angles | 90.00, 97.49, 90.00 |
Refinement procedure
| Resolution | 55.670 - 1.910 |
| R-factor | 0.1513 |
| Rwork | 0.148 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kmd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.597 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.670 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.090 | 0.639 |
| Number of reflections | 20919 | 20919 |
| <I/σ(I)> | 22.3 | |
| Completeness [%] | 99.7 | |
| Redundancy | 7.5 | |
| CC(1/2) | 0.893 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 281 | 1% w/v Tryptone, 0.05M HEPES sodium pH7.0, 20% w/v Polyethylene glycol 3350 |
