6LPC

Crystal Structure of rat Munc18-1 with K332E/K333E mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 18-ID
Synchrotron site	APS
Beamline	18-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 41
Unit cell lengths	80.475, 80.475, 247.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.072 - 3.402
R-factor	0.2834
Rwork	0.281
R-free	0.33060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3puj
RMSD bond length	0.005
RMSD bond angle	0.834
Data reduction software	HKL-3000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.520
High resolution limit [Å]	3.400	3.400
Rmerge	0.070	0.593
Rmeas	0.078	0.658
Rpim	0.033	0.281
Number of reflections	21586	2162
<I/σ(I)>	25.2	2.2
Completeness [%]	100.0	100
Redundancy	5.2	5.4
CC(1/2)		0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	289.15	4 M ammonium acetate, 0.1 M sodium acetate trihydrate