6LOS

Crystal structure of mouse PEDF in complex with heterotrimeric collagen model peptide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-22
Detector	RAYONIX MX-225
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	95.378, 147.773, 91.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.689 - 2.476
R-factor	0.2282
Rwork	0.223
R-free	0.28210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1imv
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.510
High resolution limit [Å]	2.470	6.700	2.470
Rmerge	0.115	0.044	0.857
Rmeas	0.124	0.048	0.934
Rpim	0.046	0.018	0.366
Total number of observations	157108
Number of reflections	22356	1268	876
<I/σ(I)>	6.7
Completeness [%]	95.6	99.8	75.3
Redundancy	7	6.7	5.7
CC(1/2)		0.998	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	90 mM CAPS pH 10.5, 1080 mM Sodium phosphate, 720 mM Potassium phosphate and 180 mM Lithium sulfate.