6LOH
Crystal structure of the catalytic domain of human ubiquitin ligase AREL1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-12-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 150.231, 150.231, 342.645 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.223 - 3.207 |
Rwork | 0.211 |
R-free | 0.22490 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.625 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.207 | 3.250 |
Rmerge | 0.119 | 0.628 |
Rmeas | 0.141 | 0.742 |
Rpim | 0.071 | 0.381 |
Number of reflections | 38276 | 1756 |
<I/σ(I)> | 9.6 | 2.06 |
Completeness [%] | 98.0 | 98.9 |
Redundancy | 3.4 | 3.5 |
CC(1/2) | 0.979 | 0.635 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.4 | 289 | 0.1M Sodium phosphate pH 6.4, 0.2M Potassium chloride, 2.25M Sodium chloride |