6LO2
Crystal structure of EED in complex with EZH2 peptide and compound 11#
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.310, 178.180, 50.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.090 - 2.210 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qxv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data scaling software | Aimless |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 178.180 | 2.210 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.166 | 0.612 |
| Number of reflections | 43322 | 400 |
| <I/σ(I)> | 9.1 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M TRIS pH 8.0, 15.6% PEG 8000 |
