6LNK

Candida albicans Fructose-1,6-bisphosphate aldolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	160.475, 56.758, 82.388
Unit cell angles	90.00, 95.85, 90.00

Refinement procedure

Resolution	39.910 - 2.639
R-factor	0.2011
Rwork	0.198
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b57
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.910	2.652
High resolution limit [Å]	2.639	2.639
Rmerge	0.068	0.572
Number of reflections	21939	1179
<I/σ(I)>	10.05
Completeness [%]	98.2
Redundancy	3.9
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	100 mM Hepes pH =7.5, 11% (w/v) PEG 3350