6LNG
Rapid crystallization of streptavidin using charged peptides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.293, 80.829, 132.648 |
| Unit cell angles | 90.00, 99.85, 90.00 |
Refinement procedure
| Resolution | 37.088 - 1.800 |
| R-factor | 0.183059438376 |
| Rwork | 0.181 |
| R-free | 0.21863 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ry1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.124 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.100 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.064 | 0.390 |
| Number of reflections | 78148 | 5742 |
| <I/σ(I)> | 13.91 | 3.1 |
| Completeness [%] | 99.7 | |
| Redundancy | 3.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | 0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 30% w/v polyethylene glycol monomethyl ether 5000 |
