6LNA
YdiU complex with AMPNPP and Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-11-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 112.430, 73.541, 122.649 |
| Unit cell angles | 90.00, 102.49, 90.00 |
Refinement procedure
| Resolution | 27.442 - 1.701 |
| R-factor | 0.1426 |
| Rwork | 0.140 |
| R-free | 0.18570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6iii |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.1_3469) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.442 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.094 | 0.397 |
| Rpim | 0.053 | 0.221 |
| Number of reflections | 97925 | 97925 |
| <I/σ(I)> | 12.3 | 5.3 |
| Completeness [%] | 91.5 | 96.4 |
| Redundancy | 15.1 | |
| CC(1/2) | 0.991 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 18% PEG8000 0.2M calcium acetate PH6.5 0.1M Sodium cacodylate trihydrate |
