6LLZ

Crystal Structure of Fagopyrum esculentum M UGT708C1 complexed with UDP-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	98.914, 143.757, 69.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.006
R-factor	0.1696
Rwork	0.168
R-free	0.20150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2acv
RMSD bond length	0.008
RMSD bond angle	1.191
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.059	50.000	1.970
High resolution limit [Å]	1.940	5.260	1.940
Rmerge	0.089	0.068	0.363
Rmeas	0.098	0.075	0.427
Rpim	0.040	0.032	0.218
Number of reflections	69452	3823	239
<I/σ(I)>	9.5
Completeness [%]	94.4	96.3	6.6
Redundancy	6.3	5.7	2.7
CC(1/2)		0.994	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M ammonium sulfate, 0.1M MES pH 7.0, 26.5%(w/v) polyethylene glycol 5000, 26%(w/v) benzamidine hydrochloride, 5mM UDP-glucose