6LKJ

Two-component system protein mediate signal transduction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	52.164, 67.157, 87.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.235 - 2.004
R-factor	0.1968
Rwork	0.195
R-free	0.22940
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.525
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	REFMAC
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.110	0.483
Rmeas	0.124	0.565
Rpim	0.054	0.287
Number of reflections	21110	2042
<I/σ(I)>	11.05	2.82
Completeness [%]	99.3	100
Redundancy	4.9	3.8
CC(1/2)	0.995	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1M Tris pH 7.5-7.9, 21% PEG 3350