6LK0

Crystal structure of human wild type TRIP13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	277.15
Detector technology	PIXEL
Collection date	2016-12-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97778
Spacegroup name	P 65
Unit cell lengths	90.762, 90.762, 114.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.280 - 2.600
R-factor	0.2003
Rwork	0.197
R-free	0.26490
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.560
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.082	0.054	0.883
Rmeas	0.086	0.057	0.929
Rpim	0.027	0.018	0.287
Total number of observations	167512
Number of reflections	16552	860	819
<I/σ(I)>	8.2
Completeness [%]	100.0	100	100
Redundancy	10.1	9.5	10.4
CC(1/2)		0.998	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	100mM Bicine/Sodium hydroxide pH 9.0; 10%(v/v) MPD_(+/-)-2-Methyl-2,4-pentanediol