6LJR

human galectin-16 R55N/H57R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	55.923, 34.405, 67.995
Unit cell angles	90.00, 102.25, 90.00

Refinement procedure

Resolution	19.330 - 2.000
R-factor	0.1666
Rwork	0.161
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ljp
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.330	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.039	0.124
Number of reflections	8674	647
<I/σ(I)>	16
Completeness [%]	99.4
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG