6LJ9

Crystal Structure of Se-Met ASFV pS273R protease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	60.165, 83.280, 108.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.769 - 2.307
R-factor	0.1929
Rwork	0.191
R-free	0.22020
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.843	2.200
High resolution limit [Å]	2.079	2.079
Number of reflections	21872	19824
<I/σ(I)>	10.67
Completeness [%]	100.0
Redundancy	7.04
CC(1/2)	0.999	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	289	0.2 M Lithium sulfate monohydrate, 0.1 M Tris, pH 8.2, 23% w/v Polyethylene glycol 1,500